We are working with Gram positive Actinimyces species which have 65 % GC content in their genome. Because we are not allowed to bring live bacteria from abroad into our country so we want to import dead/inactivated cells, then extract DNA and do whole genome sequencing.
We have difficulties in choosing the best method/solution to preserve bacterial cells so that we can extract DNA later. We have several suggestions to use RNA later, 70% EtOH, 95% EtOH. Does anyone know what the best preservation method we should use. Thanks
Ethanol is a very good option because it will kill the bacteria and preserve the DNA. I'd recommend 95%. 70% is actually better for surface decontamination in a microbiology lab, but 95% is best for preserving DNA. You can easily remove the EtOH back at the lab by pelleting and removing the supernatant and leaving it to dry. RNA later can be a pain because it is viscous and it is harder to pellet from it.
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