Lucas Vajko

5 Questions 1 Answers 0 Followers

Questions related from Lucas Vajko

H&E Black Granules - Necrosis?

Just curious if what I am seeing in this H&E image of a treated bioprinted liver is necrosis. The pattern looks different than what is online.

04 November 2023 882 1 View

Proper Alcian Blue Stain in 3D printed Liver tissue?

Just wanted to see if there were any opinions on the Alcian Blue histological stain in for a 3D bioprinted liver model. Alcain blue was utilized here to determine if the proper production of...

04 November 2023 2,346 0 View

B-Cell - Ramos Cell - Brightfield, Immune, and/or Morphology Question?

Hello, Just a question from any immunologist or cell culture expert who would know what the black granules that look sort of like eosinophilic granules are in the cultured immortalized B-cells....

04 November 2023 1,955 1 View

CCL4 Carbon Tetrachloride In Vitro Toxicology - Bubble Formation or Plastic Degradation?

Upon adding CCl4 (Carbon tetrachloride) to my in vitro sample, I noticed bubbles or maybe the plastic being degraded at the bottom of the 12 well plate. The concentration used was 4mM, which is in...

23 October 2023 5,747 1 View

Curious about a proper protocol for albumin collection over long periods of time?

I am mostly confused regarding media changes and how that affects accumulation of albumin or other soluble factors secreted into the media. If you were collecting albumin over a two week time...

15 September 2022 8,412 0 View