B cell isolation from mice spleen using Meltinyi negative kit?

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Alois Palmetshofer

The most critical thing is to treat the cells very gently in order to avoid aggregates and to not overload the beads.

Vishal Khairnar

In addition to above comment, try not to wash the column for long or 2-3 times to get more yield. With one wash you can always achieve more than 90-95%.

Xinjun Wu

Thank you.