Automatically counting dots in ELISPOT?

More Øyvind Steinsbø's questions See All

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Adam E. Snook

We use FIJI (https://imagej.net/Fiji) for a lot of automated image processing and counting. There are pre-built macros for many common processes and ELISpot is likely one of them [we use Immunospot for ELISpot so I don’t have a specific FIJI macro recommendation for that].

Øyvind Steinsbø

Thanks for good answer, Adam E Snook!

Sun Yung

How are you imaging your ELISPOT?

What size plates for you use? How many plates do you normally count?