@authors: How far are you with the project? Any results / documents ready to share?

More Maira Magnani's questions See All

Autodock Vina docking?

I have a protein. It's binding site are not known hence I used castp for the prediction. I set the grid box in autodock vina and performed the docking with a ligand. However, when I see the...

04 July 2023 3,332 3 View

What cell line should I choose for citotoxicity assays?

I need to perform cytotoxicity studies with an isolated substance for a future formulation with oral administration. However, I don't know which cell lines to choose to test cell viability......

05 May 2023 3,915 4 View

Calling all zooplankton researchers: please help us identify this mystery jelly!?

These organisms were sampled at the ocean surface within the North Pacific subtropical gyre with a manta net, and we have not been able to identify what they are. Small squares in the background...

23 March 2023 5,705 0 View

Which active component in a green tea act has highest anti-inflamatory effect?

green tea and its benefits in osteoarthritis condition.

05 November 2022 6,809 2 View

Need help in making sodium silicate solution (Na2O = 7.5–8.5 %, SiO2 = 25.5–28.5 %)?

I want to create Sodium silicate solution (Na2O = 7.5–8.5 %, SiO2 = 25.5–28.5 %) by dissolving silica beads (SiO2) and sodium hydroxide (NaOH) in water. I am an environmental scientist and my...

27 August 2022 3,741 1 View

I have to prepare a fibrous sample of chicken feathers for FTIR analysis but It cant be grinded finely. What should i do?

I have to prepare a fibrous sample of chicken feathers for FTIR analysis but It cant be grinded finely. What should I do? To be exact, these are magnetized chicken feathers. Also i am afraid that...

18 August 2022 5,285 4 View

Bi directional permeability assay Caco-2?

Hi everyone, we performed Caco-2 Permeability (Bi-directional) assay for different compounds, and when we measured Papp (B-A) and Papp (A-B), in most of the tested compounds we found only Papp...

19 July 2022 7,890 1 View

Has anyone experienced high cell death during monoclonal cell line isolation after cells have been CRISPR-edited?

Hello! In order to isolate a reporter line, NPCs were electroporated with a CRISPR construct. The cells grow well as polyclonal, but when cultivating them as single cells for isolation of...

11 January 2022 2,097 4 View

After generating crRNA+tracrRNA heating at 95C, can it be maintained at -20C and be used in future experiments? Or crRNA+tracrRNA needs to be fresh?

I only have used sgRNAs. For the first time I used crRNA+tracrRNA and I kept what was left in the -20C. Can I use it again or is it better to prepare fresh crRNA+tracrRNA? Thank you :)

24 November 2021 1,586 0 View

How to initialize the state in Unscented Kalman Filter for Parameter Estimation?

I am implementing an unscented kalman filter for parameter estimation and I found its performances strongly related to the initialization of the parameter to estimate. In particular, I don't...

18 October 2020 3,664 4 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

How to convert a privately loaded document into a public document?

I attempted to make a privately uploaded text public but a window appeared that said an error occurred. There was no explanation provided as to why there was an error or what might be done to...

05 August 2024 8,025 7 View

How do living organisms play a role in the water cycle and why is nonpoint source pollution potentially more harmful than point source pollution?

01 August 2024 7,061 2 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Are the apoptotic cells is positive for γH2AX ?

It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...

28 July 2024 7,983 2 View

What role do microorganisms play in the cycling of matter and role of microbes in the biogeochemical cycle?

25 July 2024 3,096 2 View

How does the digestate compare to other organic amendments regarding quality and performance in different countries?

Experts are welcome to share their experience country-wise so that we can make a nice document listing all the country-wise experiences. ? How does the digestate compare to other organic...

18 July 2024 5,225 3 View

How to cacualte the creep life of polycrystal with CPFEM?

I saw a paper(PHAN V-T, ZHANG X, LI Y, et al. Microscale modeling of creep deformation and rupture in Nickel-based superalloy IN 617 at high temperature [J]. Mechanics of Materials, 2017, 114:...

16 July 2024 8,436 0 View

What kind of t-test can I use if I have two variables measured using the same raw data?

I plan to perform a t-test between risk index and leakage values to know if there is a significant difference between leakage values of low, medium, and high risk pipes. However, the data I have...

10 July 2024 6,199 3 View

Is it appropriate to apply the COSMIN guidelines for evaluating the quality of tools used to assess acute postoperative pain?

Hi, I'm a graduate student, college of nursing, chungnam national university in Daejeon. The topic of my thesis is "Quality Assessment of Tools for Evaluating Acute Postoperative Pain." I have...

10 July 2024 3,048 1 View

Emad Rabiei Hosseinabad

Maira Magnani , I have published a paper about LCA of solid waste and their comparison using MCDM ( multi-criteria decision making), that you might be interested in.

Link to my publication: https://scholar.google.com/citations?user=MSlA0HMAAAAJ&hl=en&authuser=2#d=gs_md_cita-d&p=&u=%2Fcitations%3Fview_op%3Dview_citation%26hl%3Den%26user%3DMSlA0HMAAAAJ%26authuser%3D2%26citation_for_view%3DMSlA0HMAAAAJ%3Ad1gkVwhDpl0C%26tzom%3D360

Maira Magnani

Thank you very much!

Mehdi Aliehyaei

You can also focus on extended exergy analysis