I will be propagating MS2 with E. coli. According to ATCC, a #271 broth is recommended for this. This consists of (for one liter): 10g Tryptone, 1g Yeast Extract, 8g NaCl, 15g agar, plus glucose, CaCl2 and thiamine. I was planning to buy the components separately, but it may make more fiscal sense to buy LB broth. The first portion of this is very close to a Lennox LB broth that I have found. However the main difference is that the yeast is in a higher concentration than the 271 medium (5g/L instead of 1g/L).
So, my question is, will this elevated yeast concentration have a measurable effect on E. coli or MS2 growth. I plan to quantify MS2 using double layer agar assay if that has matters at all.
Hello, you can find your answer by experimental way. You need to validate the new media which mean the comparison of selectivity, sensitivity, of both media also grow promotion test and you can compare the obtained data by t-test.
Good luck.
E. coli will likely grow a bit faster in LB relative to 271. However phage labs have often used slightly less rich medium (like peptone or tryptone broth). The slightly slower growth sometimes yields larger plaques or higher titers in liquid. Having said that, I doubt you will see a significant difference and I've used LB frequently in lieu of tryptone broth.
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