At what temperature should crude lipase enzyme from bacterial source should be stored before and after lyophilization?

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,365 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Is it possible to improve the reforming section of the Haber-Bosch process without using air separation units?

A typical reforming section in a large-scale Haber-Bosch process (Figure 1) comprises two reactors. A mixture of methane (CH4) and steam (H2O) is fed into a primary reformer to produce syngas via...

01 August 2024 7,051 5 View

Why my gel electrophoresis has smearing?

Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...

22 July 2024 9,761 5 View

Appearance of crystallization peak in heating cycle of DSC test?

For which types of polymers, the crystallization peak in heating cycle of DSC test can be observed and for which types cant? In another word, when this exothermic crystallization can be observed...

18 July 2024 348 3 View

Would anyone care to recommend an inexpensive waterproof serviceable (e.g., batteries can be changed) temperature logger?

I've been using Hobo pendants for years but they are up from less than $50 a few years ago to over $100, and I'm in need of an alternative.

08 July 2024 7,164 1 View

Is it possible to define exothermic processes in finite element software like COMSOL? If yes, how can this be done?

We aim to study an exothermic process and evaluate its temporal changes. Could you recommend suitable software for this purpose and explain how to conduct this analysis? What specific data is...

30 June 2024 8,413 1 View

How to prepare Gold nanoparticle sample for XRD?

We have been lyophilizing several nanoparticles like iron oxide, cerium oxide to get powder sample. However, for gold nanoparticles and any other NPs with gold, they turn sticky after lyophilizing...

26 June 2024 9,171 3 View

How precisely one can do impedence or diielectric studies of high Curie temperature material (i.e., KNN based materials) with silver electroding?

Will silver coating be able to hold such a high temperature? And will there be any effect of coating thickness?

23 June 2024 8,550 0 View

How can grower manage temperature at mushroom culture house?

From my knowledge- mushrooms are highly sensitive to temperature. How can growers manage suitable temperatures?

22 June 2024 7,346 0 View