I am preparing the NAP Buffer described in Camacho-Sánchez et al., 2013. The protocol calls for dissolution of 700 grams of Ammonium Sulfate in 1L of water. This step is described as "taking hours" and should be done at "low to moderate heat", but I've had the buffer on a hotplate stirrer for more than 16 hours at 55°C and it still doesn't dissolve completely. Has anyone else had this problem? What temperature range do you use? How long does it usually take you?

Thanks!

More Natalia Kazalinsk's questions See All
Similar questions and discussions