Natalia Kazalinsk

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Questions related from Natalia Kazalinsk

What tool/program do you use for taxonomic assignment of DNA obtained through Nanopore sequencing?

I'm looking to do taxonomic assignment for DNA sequenced with Nanopore. I have ITS and 16S amplified from soil DNA, and the quality is pretty good after filtration. What tool or program do you use...

16 July 2025 6,363 3 View

What is the most effective HMW DNA extraction method for fungi?

Hello, I need to extract HMW DNA from Botrytis, I've heard conflicting information on whether it is preferable to use biomass obtained from liquid cultures or from plates, whether to...

23 April 2025 4,283 1 View

At what temperature and for how long should I mix Camacho-Sánchez NAP Buffer for it to dissolve?

I am preparing the NAP Buffer described in Camacho-Sánchez et al., 2013. The protocol calls for dissolution of 700 grams of Ammonium Sulfate in 1L of water. This step is described as "taking...

19 March 2024 4,795 1 View

I can't see my purified PCR product in an agarose gel?

I successfully amplified fungal ITS from soil samples, however after running the purified PCR products in an agarose gel they are barely visible and don't look like defined bands but rather...

11 March 2024 8,462 2 View

Why would you want to use spores to identify arbuscular mycorrhizae when you can sequence them?

I'm working with Podocarpaceae rootlet nodules and want to identify the mycorrhizae inhabiting them. Wouldn't sequencing with appropriate primers be the most ideal method for this? There's no...

12 February 2021 2,611 17 View