How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

Should absorbance be between 0 and 1 for ELISA?

Hello, I am running a sandwich-type ELISA for which the readout is absorbance at 450nm. The ELISA measures protein binding which I am screening for compounds to inhibit. The 570nm...

05 October 2020 9,813 6 View

Is it possible to calculate Absorption co-efficient or Absorbance of a dye theoretically?

I am working on a Luminescent Solar Concentrators (LSC) using dyes such as Coumarin 1, Coumarin 153, and Perylene Green Ph-probe 720-5.5. I need to calculate the Mean-free-path (MFP) for the...

18 August 2020 9,804 3 View

How I can create neural network model for simultaneous determination of mixture of two drugs?

I want to help me in creating neural network for simultaneous determination of two drugs. I make 26 mixture of the two drugs this is my inputs data, the spectrum of the mixture of the two drugs...

19 May 2020 10,005 3 View

Does my TMB substrate significantly affect my ELISA sensitivity?

Hello, I am currently optimizing an absorbance-based ELISA assay. The ELISA is a sandwich-type, with the final component being a secondary antibody fused to HRP. I then use Turbo-TMB to obtain...

10 May 2020 4,017 6 View

Is there any discussion about absorption (absorbance) changing in DRS analysis?

Dear colleagues, I had synthesized silver-phosphate based photocatalyst material with the addition of PEG (various PEG concentration and chain length). When I analyzed using Diffuse Reflectance...

17 March 2020 9,366 3 View

Competive ELISA - Standard curve issue?

Hello, I am using an in-house competitive ELISA for the detection of a small peptide in the various samples (plasma, cell culture supernatants etc.). For the method's needs, polyclonal rabbit Abs...

01 February 2020 838 3 View

Is it possible for MWCNT to have some kind of solvatochromism effect?

I've been comparing the UV-Vis spectrum for oxidized MWCNT and the same non oxidized material in a few organic solvents (tetrahydrofuran, dimethylformamide, toluene and chloroform) and for DMF,...

19 January 2020 2,264 2 View

Is it possible to increase/decrease band gap of metal oxide thin film due to thermal annealing?

I got confused by the shift of the absorbance region which lead to the shift of bandgap.

05 January 2020 8,978 4 View

How to determine drug amount within nanoparticle properly?

I have a drug-loaded np system (simvastatin and oryzanol) but keep getting negative absorbance with uv vis spectrophotometer. The steps I'm using is: 1. Drugs dissolved in NP (100 ppm) by stirrer...

31 December 2019 4,480 3 View