At 425 nm a 1.2 x 10-3 M solution of compound Q has Absorbance = 0.879. Another solution of Q is prepared by diluting 25.00 mL of the 1.2 x 10-3 M ?

06 July 2023 1 7K Report

At 425 nm a 1.2 x 10-3 M solution of compound Q has Absorbance = 0.879. Another solution of

Q is prepared by diluting 25.00 mL of the 1.2 x 10-3 M Q to a total volume of 50 mL. What is the Absorbance of the new solution at 425 nm?

Paul Milham

If the relation between concentration and absorbance is linear, halving the concentration will halve the absorbance

Badges
Science topic

More Yahya Alsayaad's questions See All

Do you think can be any Uranium bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about Uranium ore deposits in world.

11 August 2024 6,720 0 View

Do you think can be any diamond bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about diamond ore deposits in world.

11 August 2024 2,167 1 View

What is the difference between mathematical R^4 space and physical 4D unit space?

We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...

10 August 2024 6,678 14 View

If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?

10 August 2024 8,198 5 View

Controlling for pupil light reflex when analyzing pupil size time course?

I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...

10 August 2024 3,229 0 View

What are a “Farmers Producer Organization” (FPO) and its essential features?

10 August 2024 477 5 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

How to get moment output in Abaqus Standart?

I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...

08 August 2024 4,831 4 View

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

08 August 2024 8,162 0 View

How to calculate my FRAP Assay?

Y=0.0011x+0.1031 (Standard curve eq) Chamomile sample 20mg/mL Absorbance(y)= 1.16 x=960.82(µg/mL) Chamomile sample 10mg/mL Absorbance(y)= 0.80 x=633.55(µg/mL) Tridax sample...

01 June 2024 7,843 0 View

How to calculate the total phenolic contents (TPC) in plant extract sample?

How do I calculate the total polyphenol content in mg GAE/g. from the extract? Following is my data: Sample extract(stock): 0.01 gram in 1 ml Equation: y= 0.0078x - 0.0679, R2 =...

27 May 2024 1,911 2 View

Validation of the formula for beta-carotene estimation?

For the estimation of beta-carotene by acetone extraction method using petroleum ether and sodium sulphate at 453 nm. it is known to all that the extinction coefficient of beta-carotene at 453 nm...

16 April 2024 3,739 1 View

Help: Troubleshooting Metash UV-Vis (MetaSpec Pro), Negative Absorbances <230 nm?

Tl;dr - As of last week, the UV-Vis instrument at my job only records negative absorbance below 230 nm for any sample when zeroing methanol to correct for solvent. Attached spectra of the same...

03 April 2024 5,271 5 View

Has anyone experienced a significant A after 15 min. of thiol disulfide exchange reaction and then no A could be detected?

In thiol-disulfide exchange reaction, pyridine-2-thione is expected to be released as the reaction proceeds giving yellow color to the medium, and the Absorbance should increase and then become...

15 January 2024 689 0 View

Why is the absorbance of the egg/bovine serum albumin denaturation assay taken at 660 nm?

Denaturation assessments on egg albumin and bovine serum albumin suggest a link between tissue protein denaturation in living organisms and the onset of inflammatory complications. In this assay,...

09 January 2024 8,773 2 View

How to calculate Extinction Coefficient for Assay?

I am using SpectraMax 190 Microplate Reader kinetics (Endpoint) for an Assay that automatically corrects Optical density measurements to a 1-cm pathlength with PathCheck Technology. While using...

03 January 2024 5,867 0 View

How can I estimate gas concentration in ppm?

Hello everyone, can anyone pls guide me on the following topic: I have an absorbance data (log10 Io/I) of an ablated smoke sample measurement performed with a gas cell. I have identified some...

02 January 2024 1,645 1 View

In spectrophotometry readings, does anyone know the difference between Absorbance and Standard Concentration readings?

I'm working with a Thermo Scientific GENESYS 10S Series UV-Visible Spectrophotometer. I took readings of a cell culture in two different modes: Absorbance (595 nm) and Conc/Std (Factor = 0.745,...

16 October 2023 2,898 1 View

Non stable Absorbance value using Perkin Elmer lambda 40 ?

Hello researchers, Please, Could anyone help me to know what is going on with my spectrophotometer UV-VISIBLE Perkin Elmer lambda 40. The balayage working perfectly (190-1100nm) But when using a...

01 October 2023 6,131 3 View