How can we assess the presence and amount of T-cells in the freshly removed skin tissue after surgery or punch biopsy? Also, if we freeze the skin tissue at -20C for a long time, after thawing to warm PBS or water, how can we make sure we have T-cells especially CD4 and CD8 T cells in the extracted frozen skin tissue? Does skin have to go in a specific solution or it has to be assessed for the specific markers ?
Attaching papers for your reference. It is quite common to look for lymphocytes (CD4,CD8) from fresh punch biopsy. Not much knowledge about frozen biopsies.
Attaching papers for your reference. It is quite common to look for lymphocytes (CD4,CD8) from fresh punch biopsy. Not much knowledge about frozen biopsies.
Yes, you are correct it is common to assess lymphocytes in the skin. I thank for the paper you have attached. It is quite interesting one Jitendra Kumar Shandilya
I would suggest to isolate cells before freezing. Cellular yield frozen tissue will be very low than the fresh. Better if you isolate cells and then freez the cells in freezing media.
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