Hello everyone,
I have known that Michel's Transport Medium (which is used for IMF studies), is a good medium to preserve immunoantigenicity of fresh skin but up to 5-7 days.
Dulbecco's Modified Eagle's Medium (DMEM) with high glucose is a supporting media for the growth of cells, in this case skin.
I am wondering is there any other medium or a protocol to maintain the skin conditions of ex vivo skin for a longer period of time, 1 month to 3 months?
As far as I understand there is no publication that have shown successful maintenance of ex vivo skin model for an extensive period of time regardless of the culture condition
There was once I asked this question to an expert in this field and his reply was to make the skin explant as thin as possible in order to extend the viability of the skin. Nonetheless, the lack of blood perfusion/vascular network is the main reason why it cannot be maintained for months in ex vivo culture
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