ASO to upregulate gene expression?

More Satyanarayana P.M.'s questions See All

How to add my publications to my account?

I have several articles with two names Satyanarayana Murthy Pusuluri and PSN Murthy. How do I get all of them into my account

11 January 2024 7,847 1 View

Could somebody kindly recommend a highly regarded research article pertaining to the fundamental applications of lead-free perovskite quantum dots?

I am currently seeking lead-free perovskite quantum dots for implementation in optoelectronic devices. I have conducted an extensive search of academic papers, but I have yet to find a suitable...

03 October 2023 1,819 3 View

Dead cell clumps of LN caps?

Hi I have been working with LN cap cells for 2 months now.As I understood they are semi adherent cells and handling is very tricky.Somehow when I seeded my cells in 6 well plates I saw the clumps...

30 July 2023 8,453 0 View

How to find the locus tag of a gene?

How to find the locus tag of a gene? I downloaded a FASTA file & GFF file of my gene of interest. I want to know the locus tag.

06 March 2023 9,311 2 View

Do pesticide-degrading bacteria tolerate any concentration of pesticide?

Do pesticide-degrading bacteria tolerate any concentration of pesticide? Please share the limit of pesticide conc. used in lab studies. A review paper can be useful! Thanks in advance.

23 February 2023 2,818 3 View

On what basis, do we choose to tag protein with GFP at either the C or N-terminal?

If I were to express a protein with GFP, then where should I fuse the GFP gene, at which end? Thanks in advance!

12 February 2023 9,360 4 View

Could you please give valuable suggestions on my Project?

I just started a project entitled "Implementation of Village Knowledge Centres (VKCs) in Kerala towards the achievement of Sustainable Development Goals(SDGs): a feasibility study". I expect novel...

26 January 2023 1,808 6 View

What is the correct expression of calculating the "Meshedness coefficient" of a network ?

expression for Meshedness coefficient

20 February 2022 2,444 2 View

Please suggest problem in the Autodock molecular doking ?

While doing autodock for my moleucle which contains a bromine atom, after completing the docking process, while i am analysing the output poses, always the bromine atom is out of the moleule with...

02 December 2021 5,374 13 View

Are revised manuscripts submitted to top journals reviewed by the same set of reviewers?

Occasionally, it can be noted that revised manuscripts are sent to a new set of reviewers who have a different opinion on the earlier manuscript. Trying to understand the normal practice by top...

16 June 2018 7,149 7 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,081 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,537 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,881 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,618 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 806 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,365 3 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,129 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,786 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 500 2 View