Fractional derivative users, are you on the same page with me on the validity of the fractional order parameter, say alpha?

Taking a fractional ODE problem as an example. It has come to my attention that if the fractional order parameter is in the interval (0,1], many only presents results for alpha=0.9, 0.99. I saw...

08 February 2021 9,671 3 View

Is it time to have a universal research paper format for all journals?

In the scientific world, some terms and practices are universal. Look at botanical names that are uniform in all nations for example. I think global standardization should apply to research paper...

15 December 2020 6,936 12 View

Can anyone help with how to dissolve Iodonitrotetrazolium salt for MIC?

I got the reagent without the document on how to dissolve it for specific assays

11 September 2020 8,074 3 View

What's the best way to take clear pictures of your field in light microscopy?

When you get a clear view of your organisms and you need pictorial documentation, it becomes problematic. What are your experiences and suggestions?

23 August 2020 3,399 3 View

How do I enter intersection values in Venn diagram on Microsoft Word?

We have created a Venn diagram on MS Word and entered values into the main circles, but we have no way to enter values into the intersections. How do we go about this?

17 August 2020 8,890 6 View

Is it compulsory to add urea salt to the urease test medium before inoculating and incubating?

Is the addition of urea salt compulsory in carrying out urease test? The salt is often not sterile in storage, and it cannot be autoclaved because it is denatured by high heat. I'm afraid this may...

01 April 2019 3,445 1 View

What institution does the author state when publishing an article, if they have left the institution where they carried out the research?

Do they state their current institution, or the institution where they carried out the research, or both?

08 March 2019 2,538 3 View

Aptitude-rated iq versus academic performance of students

Correlation between IQ measured by aptitude performances and Academic Performances: it has been observed that Academic grade alone does not imply suitability for work as well as performance; hence...

30 August 2012 1,909 2 View

Fairness of current research-publishing practices

A researcher spends a lot of money conducting a study and when the results are out, THEY have to pay to get their work published by a journal. Some Q1 journals can charge up to $6000 to publish 1...

01 January 1970 1,513 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

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02 March 2021 3,060 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View