How to resolve a corrupted .odb file?

Hello. I run my Abaqus simulation in the supercomputer and once it's done, I copied the result (odb file) into my local computer using winSCP. But I faced a problem to read that file (see...

08 February 2021 6,198 3 View

H-index in the profile of Scopus?

Does the H-Index for researchers appear on the Scopus website without activating or doing anything by the researchers?

17 November 2020 3,849 5 View

Is there relation between ABO groups and anemia?

dear Colleague I have research with my students about ABO groups and Anemia. Is there relationship between both of parameters? what your opinion and do any have references because we looked for...

09 March 2020 8,528 11 View

What is the best strategy for specific scientific research?

The scientific basis for choosing the idea of research with the lowest probability of expected errors.

30 January 2020 8,525 15 View

What are the factors influencing on morphology and physiology of freshwater microalgae ?

Effects of different media composition, light intensity and photoperiod on the morphology and physiology of freshwater microalgae Ankistrodesmus falcatus - potential strain for biofuel production

02 December 2019 8,658 3 View

How to prepare activated carbon from sewage sludge in a simple way?

This is my graduation project subject and I want your advise and recommendations of the experimental procedure.

07 September 2019 5,738 4 View

How to analyze the qualitative interview data?

How can we analyze the qualitative data from a semi structured interview? and how much the sample size needed for ethnographic qualitative research?

13 April 2019 585 11 View

Calculating Stack minimum value!

How can I add a condition in ERDAS Modeler to calculate Stack statistics withing values above zero only??

02 August 2017 7,111 1 View

If I have a big sample size, is it acceptable to assume that data is normally distributed?

24 March 2017 1,271 19 View

How can I deal with dataset attributes that have binary values?

25 January 2017 2,656 9 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View