Are we still under the influence of Western ideologies therefore making us "slaves" to their directives in all aspects of development?
Following...
i do not think so
Yes
Yes but in a canning with the subtle name neocolonialism where our masters determine everything for us including trade and ill virtues such as lesbianism, gay and the likes which were not part of our culture.
Why are some students of the basic school not skilled using computers?
04 May 2018 3,085 5 View
Why do some parents impose on their children not to do certain programmes in school?
04 May 2018 7,267 4 View
Must the child be trained to learn art history?
04 May 2018 3,077 5 View
How do we encourage kids who wants to pursue art in the future as teachers?
04 May 2018 6,509 4 View
What makes an informal educational artist gain recognition?
04 May 2018 6,045 2 View
Can informal education artists be trained to have a formal education?
04 May 2018 1,354 3 View
Which type of network is used to connect digital devices within a half-mile or 500-meter radius?
04 May 2018 1,840 0 View
Is Photoshop essential for basic school students who wants to practise it?
01 February 2018 6,452 5 View
Must kids from the basic level be taught about the Adinkra symbols and their meanings?
01 February 2018 9,761 8 View
I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...
25 February 2021 8,404 3 View
I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...
24 February 2021 8,514 3 View
Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...
23 February 2021 8,609 3 View
Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...
23 February 2021 6,671 4 View
I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...
22 February 2021 9,901 3 View
I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...
22 February 2021 6,937 3 View
I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...
22 February 2021 1,777 3 View
Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...
17 February 2021 4,844 4 View
Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...
17 February 2021 1,130 6 View
I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...
12 February 2021 5,824 3 View