How to clean up an already pure protein from grwoth medium?

Hi all, I have got a protein, expressed in P. pastoris vector, more than 97% (SDS-page) pure in the culture medium after cells separation. Despite the protein expressed has a his-tag, Nichel...

04 February 2021 5,173 6 View

What is your sentiment concern the impact of Covid19 on the main industries in Eu and USA ?

Covid 19 seem to be an huge crisis factor for the economy of several country . Im looking data for a first evaluation on EU and USA economy for to understand the best action for fight this impact .

07 April 2020 5,254 10 View

Direct viral RNA detection (RT_PCR)?

Is it possible, in your opinion, to detect RNA using RT-PCR but without RNA extraction? Considering for example a pharyngel swab sample, it is possbile to do a pre-treatment in order to preserve...

29 March 2020 7,576 5 View

Parameters selection for HDBSCAN algorithm in Supervised setting ?

I would like to know if there is any kind of research on the parameter selection (minpoints) for the HDBSCAN algorithm, in the context of having a training set with only normal samples and a test...

06 March 2020 3,487 3 View

How can I solve convergence problems of a dynamic analysis of an earth dam with opensees?

Hello everyone! I'm trying to model a earth dam with opensees. To do TCL file, I used GiD ProblemTypes for 2D Slope Analysis Input File Generation. When I do dynamic analysis with small...

03 September 2019 2,508 2 View

Are ACO and PSO ranking based feature selection approaches?

Hello, I've just came across these two algorithms and I was wondering whether there are any available versions for ACO and PSO as ranking-based feature selection approaches? Any comment would be...

18 July 2019 4,528 8 View

How can I calculate the aerodynamic characteristics of horizontal tail in FLUENT?

22 May 2019 6,252 3 View

How can I overcome the 230/260 low ratio problems after DNA clean up? and why do you think i got this problems?

Hi to everybody, I am doing the cloning experiment but I have problems before performing the digestion as my clean-up products have a 260/230 ratio of 0.5. There is someone available to explain...

03 May 2019 5,354 8 View

Where can I find the right CHARMM22 parameters for my paramagnetic complexes?

Hello, I'm trying to model some gadolinium complexes through molecular dynamics using the parametrized CHARMM22 force field as described in International Journal of Quantum Chemistry, Vol. 73,...

04 April 2019 6,889 1 View

How can Principal component analysis (or factor analysis) be applied on a questionnaire score, and how can the results be intepreted?

During my research, I asked several companies to complete a questionnaire to measure they risk maturity. At the end of the questionnaire, each respondent receives a score from 1 to 5 (1=low...

26 March 2017 491 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Is the sinovac vaccine safe and effective for people over the age of 60 ?

Dear respective researchers, I was asked by my institution to be vaccinated this week using the sinovac vaccine. However, from some of the information I got, this vaccine is not recommended for...

01 March 2021 7,781 6 View

How can we obtain a modification factor for creep parameters?

I want to obtain standard creep parameters of salt rock by creep parameters of rectangular samples. Is there a modification factor to convert creep parameters of rectangular (prismatic) rock salt...

01 March 2021 7,131 1 View

Anyone found any research papers on solar furnace design calculations (for solar concentration)?

I am thinking about working on solar furnace with heliostats that concentrate sunlight and heat molten salt, but currently no luck with research. Not a lot of papers based on the subject. (check...

01 March 2021 5,023 5 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

If a given country passes mandatory 'vaccine passport rule' to go outdoors, otherwise stay at home, can it help to vaccinate all against COVID-19?

Wether vaccine passport declared not to go out of home without it may help taking COVID-19 vaccine. Can't it break individual's right to protect the community? Your comments on perspectives...

27 February 2021 5,549 23 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View