Ideal samples packaging for edible oils?

Hello everyone, I have maybe a stupid question regarding the right packaging to use when comes to send some oil samples in external labs. Do you have any advice about that? Is there a risk that...

18 January 2021 7,826 4 View

Alternative to use of ethanol in extraction process?

Dear all., I am using ethanol as part of the extraction process of Chlorella vulgaris carbohydrates. Since I am trying to scale up a bit the whole process, I should use much more ethanol, that in...

27 August 2020 1,875 5 View

Do I have to activate NR8383 rat macrophages with LPS before incubation with particles and investigation of cytokines release?

Hello everybody, I'm planning to investigate the in-vitro release of different markers (CXCL10, INF-a, IFN-b, 1L-1a, IL-1b, IL-6, free DNA) by NR8383 rat macrophages, upon incubation with several...

20 August 2020 8,791 1 View

Do you know if Covid-19 dataset is available somewhere?

I'm searching for a numerical dataset about the virus. I found only daily statistical data but i would like access to single patients data. Does anyone know about it?

09 March 2020 6,626 85 View

How do I report stats for a zero inflated negative binomial model?

I need to report my statistical findings in a paper but I am struggling to report the output from R. how do I treat the two sections (Count model coefficients and Zero Inflation model...

28 January 2020 1,422 2 View

What statistical method would you use to analyse point count data on butterfly migration?

I have a set of data to analyse on butterfly migration. Unfortunately my stats skills are not as good as my field skills! Me and my peers have counted the amount of butterflies migrating in one...

09 January 2020 8,150 11 View

How to choose a suitable journal?

Dear researchers, I know some sites that can help to choose a suitable journal to a given manuscript, namely: journalfinder.elsevier.com journalsuggester.springer.com journalfinder.whiley.com can...

13 October 2019 1,453 3 View

Buffer without monovalent salts, can I use Tris?

18 April 2018 8,432 1 View

Alternative to PBS buffer with low salt concentrations?

Hello all, I find myself in need to perform serial dilution of E.coli cells for a luminometric ATP assay. Normally for serial dilution I would use PBS, but from what I understood the...

18 April 2018 5,359 3 View

Toxicity of H2O2 against coli?

Greetings, I am currently trying to determine the toxicity of the effluents of an Electro-Fenton reactor with macrotoxicity method and colony count. The concentrations of H2O2 I calculated...

13 January 2018 1,660 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View

Measuring Total immunoglobulin whole-body of fish larvae?

Hi Dear All, I prepared the extract of fish larvae whole body with PBS. Then protein concentration of the whole-body extraction samples determined by the Folin-Ceiocalteu-Phenol method (Lowry...

17 February 2021 1,971 3 View

What is the identity of additional bands found during fish gut microbiome 16S V4 PCR amplification?

Hi, I am carrying out test PCRs for 16S amplicon sequencing on Illumina MiSeq. I am using standard V4 primers: 515F/806R. The aim is to profile the microbiome of fish guts under different...

16 February 2021 5,668 6 View

Any recommendations for methods to analyze elephant space-use (using GPS) and correlating this with changes in NDVI?

For my Masters I am looking into elephant space-use and its effect on vegetation change. I have elephant GPS collar data and was going to use Google Earth Engine to obtain NDVI values. I would...

16 February 2021 7,097 3 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View