The answer of such broad specfic subjects needs special selective well qualified scientfic persons and special unit to deal with sterotoxic injections of viral vectors like intracranial injections of adenovirys,rabies,.
Also conditional of gene manipulations.,Finally steroxic injections,and dreadd viral vectors need unit completely isolated under quarantine observations by professionals either animals handling by professionals according to the guideline of animal care and use,and get rid of used syringes in scientfic safe way according to viral vector protocl.
Ref.https://www.Jove.com> intrcranial
At our institute we take the following general precautions when handling viral vectors for stereotaxic injection of rodents:
- Prepare and handle virus solutions in a biosafety II cabinet, in a dedicated room
- Keep handling of virus sharps to an absolute minimum. (Where possible, use injection equipment that does not require loading of a virus into a needle/syringe.)
- Extra PPE in the virus room (N95 masks, double gloves recommended)
- Double-bag and autoclave all waste that comes out of a virus room (including routine waste - gloves, paper towels, etc - as well as waste likely to be contaminated)
- Decontaminate all surfaces with Virkon followed by 70% ethanol. Extra cleaning / extra precautions in virus rooms.
- 3-day isolation period for rodents injected with virus, during which they are handled only by the researcher responsible (not by animal facility staff) and only inside the virus room.
- Disposal of animal bedding by autoclaving following the 3 days.
The main hazard posed by viral vector work is from integrating viruses (eg, lentiviruses) which could theoretically increase your cancer risk slightly if they integrated into a bad place in your genome. Modern replication-defective viral vectors are very safe (AAVs in particular since they are non-integrating) and these precautions are probably excessive, although they are standard practice. If you have reason to think your virus is much more dangerous, then you should speak to your institutional biosafety advisor about appropriate risk management. I don't think a DREADD virus is likely to carry an additional layer of danger compared to the empty vector.
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