Are there size restrictions when designing primers to NGS?

Bat Chen R Avraham Lubin @Bat_Chen_Avraham_Lubin

06 June 2014 1 790 Report

I want to design primers to NGS in the illumina. Are there restrictions on the size of the overlap section or at sea level or sequence primers previously paved section?

Would appreciate help and tips!

Andrii Tarieiev

maybe some of these links will be helpful for you

http://nextgen.mgh.harvard.edu/CustomPrimer.html

http://tucf-genomics.tufts.edu/documents/protocols/TUCF_Understanding_Illumina_TruSeq_Adapters.pdf

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