What is the best assay to extract tannins from grape berry?

from ethanol extraction tanks

11 December 2016 6,291 2 View

Any suggestions for Peptide database?

11 December 2014 1,435 6 View

Are there size restrictions when designing primers to NGS?

I want to design primers to NGS in the illumina. Are there restrictions on the size of the overlap section or at sea level or sequence primers previously paved section? Would appreciate help and...

05 June 2014 691 1 View

Stable transfection of hybridoma cell line NG108-15.

I'm trying to do a stable transfection of hybridoma cell line NG108-15. I'm using FuGENE(R) 6 transfection reagent in ratios of 3:2 (reagent:plasmid) in 6 well plate and in confluence of 70%....

03 April 2013 5,523 5 View

DNA extraction from phage colony

I'm having a problem extracting DNA from phage colony for sequencing. Currently we are using the standard protocol in the manual and getting very low yield or proteins. Any suggestion?

31 December 2012 7,616 9 View

Can anyone help with C7C peptide not working?

We are trying to do a negative selection on the library before going to positive selection on our target. Unfortunately, the phage barely adsorbed into the different cells we used. Anyone have any...

10 November 2012 5,910 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View