Are there reference ranges for TNF-alpha, IL-6, IL-1 beta and LPS in human blood?

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Does anyone know why tubes with fecal (poop) samples explodes after taking them off liquid nitrogen?

We are facing explosions of falcon tubes with poop samples after taking them off liquid nitrogen. They have been stored for 2-3 months. They are not pure poop, but mixed with glycerol. What could...

26 June 2022 1,688 3 View

Any idea about cellular viability markers, which are not influenced by the mitochondrial activity and by the dynamics of the cellular membrane?

Usually I use the calcein or the MTT, but my present experiments consist in determining the quantity of mitochondrial ROS in my stressed cells and my results of cellular viability seem not...

08 December 2015 9,646 3 View

How can we make a gene correction of LRRK2 mutation on iPSC reprogrammed from fibroblasts of a parkinsonian patient ?

I should correct the LRRK2 mutation on my cells. But I hope to know is it better to do the correction on fibroblasts (before reprogramming them on iPSC) or on iPSC (after reprogramming)? And if...

11 November 2014 9,050 1 View

What structure of carboxylic acids gives the highest acidity?

Consider the monocarboxylic acid X-CO2H. X is NOT aromatic and does NOT contain heteroatoms. What is the X leading to the highest acidity in solution and/or in the gas phase?

10 March 2014 6,050 6 View

What is the best way to inhibit a gene expression: siRNA or shRNA?

I have to inhibit a gene expression within mouse embryonic stem cells for a minimum duration of 15 days.

04 December 2013 1,188 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How long will macrophages remain in the M1 state after treatment with 100 ng/mL of LPS?

In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...

01 August 2024 476 2 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

Does anyone notice the tumor regression impact of Hepa1-6 cells in HCC orthotopic mice model?

To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...

18 July 2024 9,177 2 View

Differentiation THP1 cells into M2 macrophage with IL4 and IL13?

Recently, I tried to differentiate THP1 into M2 macrophage using IL4 and IL13 (purchased from R&D). This is my protocol. THP1 was seeded into 6-well plate (10E6 cells/well). Incubate with PMA...

15 July 2024 5,153 2 View

What do I do with dried urine samples?

I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...

14 July 2024 6,724 0 View

Unexpected 35 kDa Band Observed During Expression and Purification of Dengue 3 NS5 RdRp Domain ?

Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...

13 July 2024 9,950 3 View

How long can I store mouse tissues in neutral buffered formalin for, and how often should I add fresh formalin?

My study uses C57BL/6J mice and I am required to store portions of the important organs in neutral buffered formalin (NBF). Could I potentially keep those tissues in the formalin for a few weeks...

06 July 2024 8,360 2 View

THP-1 cells completely dying with PMA, help?

Have been trying to induce THP-1 using PMA at different concentrations (range from 5 to 50 ng/mL) but after 48h all the conditions except the control are mostly dead, checked by trypan blue and...

03 July 2024 3,884 5 View

What could be the expression profile of alkaline phosphatase from lactic acid bacteria?

I have already cloned ALP gene of L rhamnosus into pET22b+ vector. Cloning is already confirmed by restriction digestion and sequencing. Cloning is achieved in BL21(DE3), Codon+, Rosetta and Lemo...

02 July 2024 5,332 3 View

Kahlile Abboud

Thank you very much! I'll look for this paper!

Francesco Fiz

I found this paper; in 23 control subjects TNF-Alpha levels were 11.2 +/- 7.31

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1533889/

Thank you Francesco Fiz!

Haley Farrie

Hello All,

Looking at the same factors for a HFCS study. Are there similar references for these ranges in mice?

-Haley

Zaida Zakaria

Thank you all...really appreciate it.

Michael Andrades

Take a look at this one: Article Serum IL-1β levels in health and disease: A population-based...

It describes IL-1b ranging from 0.3 to 1.4 pg/mL, depending on the medical condition.

Sandro Malacrida

Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays.

Todd J, Simpson P, Estis J, Torres V, Wub AH.

Cytokine. 2013 Dec;64(3):660-5. doi: 10.1016/j.cyto.2013.09.018. Epub 2013 Oct 12.

Serum IL-1beta levels in health and disease: a population-based study. 'The InCHIANTI study'.

Di Iorio A, Ferrucci L, Sparvieri E, Cherubini A, Volpato S, Corsi A, Bonafè M, Franceschi C, Abate G, Paganelli R.

Cytokine. 2003 Jun 21;22(6):198-205

Solomon Ansari

Hi, I want to know how sensitive the kit is to the minimum level to be discovered? And if the Katie's sensitivity is 6. And the answer is 1.98, is this correct?