Can I freeze and thaw staurosporine treated cells as control?

I want to set up an assay for Apoptosis and have ready my control with a "X" % positive events for Annexin V and DAPI. Can I pre-treat cells with Staurosporine, and then freeze them and keep them...

19 August 2020 6,954 4 View

Retinol as water in oil in water emulsifier?

Hi Folks, Have you ever used retinol to emulsify water inside the oil phase of a w/o/w emulsion? Retinol shouldn't be a good emulsifier as it is very hydrophobic, but it seems that the hydroxil...

14 January 2020 8,025 3 View

I have too much experimental variability in OGD procedure. Any solutions/ideas?

Hi, We are working with an in vitro model of blood brain barrier (BBB) using bEND3 cells and oxygen/glucose deprivation procedure to study the BBB damage during a cerebral ischemia. We have...

27 November 2016 1,767 3 View

Does exists some repository with bam/fastq files?

21 March 2016 5,097 1 View

How to convert vcf files generated by intersectBed bed files?

10 January 2016 8,001 2 View

Opinions and point of views regarding Biophilic Design

Hi! My name is Ana and I am a student who is studying biophilic design, I was wondering what is your opinion regarding biophilic design and LEED certificate, do you think it has a future, do you...

01 January 1970 668 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Suppose we have a banana seller cart consisting of 4 wheels, weighing about 300 kg. How much torque motors are needed to pull of push it electrically?

I am thinking of electrifying the street food carts while harvesting energy using solar panels on top.

22 February 2021 4,323 3 View

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View