On the surface of agar slant I can get enormous density of cells with high sporulation rate. In case of submerged fermentation neither the cell density nor the spore formation reaches the same level. I guess there might be some general principles in the background, and parallel, some special ones depending on the genus and species.
Sporulation media exist for some bacteria. Sporulation efficiency dpends on medium, bacteria, etc...
Sporulation is primarily stress induced. So check on what kind of stress induces sporulation in ur strain of bacteria. Nutrient, medium fluidity & temperature stress may work
As Vidyah already wrote, sporulation is stress-induced. For Bacillus sp., diminished nutrient content generally induces sporulation, as does agitation at 200+ rpm (for some species / strains).
Sporulation can be induced by reducing the amount of nutrients in the medium, changing incubation conditions, depriving them oxygen, increasing carbon dioxide in the environment etc depending on which culture is to be sporulated.
Thanks a lot, already have got some good direction of trials from all of you. Nevertheless if anyone else would have some additional idea...
Well, that is the point. We are about developing a soil inoculant containing several strains. For the sake of N2 fixing, P mobilization, plant growth promotion, stress resistance increasing, elevated immune responses etc. Species: B. megaterium (spore), Azotobacter croococcum (cyst), Paenibacillus: polymyxa, amylolyticus, macerans (additional ones as Pseudomonas, Azospirillium and Cellvibrio are non spore forming)... pretty nice "company" after my 35 years in fermentation industry dealing with antibiotics and amino acids for feed. In case of Bacitracin production the B. subtilis (licheniformis) strain automatically sporulated at the end of the process, Corinebacterium glutamicum, later E. coli were not the best :-) collecting experience about sporulation.
Hi all! I wonder whether I use Schaeffer sporulation medium to let B. cereus, B. licheniformis and B. stearothermophilus to produce spores. I had tried few times to include some mineral ions to induce the spores from these microorganisms in broth medium. Should I change to agar plates? I used endospore staining instead of phase contrast microscope to observe spores but I couldn't find anything even I grew them for 3 days. So upset about it. Could anyone can help. Thanks.
Hi Kokwai,
Depends on what amount you need. If you have to get lab scale preparation, the solid medium is a reasonable way.
To try it on Petri dishes is not a big deal of work. Let's try! On agar slant I have got enormous percent of spores.
- Badges
- Science method
- Similar topics
- Biological Science
- Ecology