22 August 2018 1 1K Report

I run a lot of qPCR plates on a regular basis. I end up spending quite a bit of that time preparing multiple serial dilutions of plasmids for each individual plate. If I try to reuse the same serial dilutions multiple times in the same day, my standard curve ends up being more unusable as the day progresses, risking not being able to use the data from a plate and having to re-run it later. Freezing the samples overnight and reusing them the next day doesn't work either.

It would save me a lot of time if someone could find a way to stabilize a serial diluted curve that could be continuously reused.

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