I mean what the molecules can activate P2X and P2Y (other than ATP) for bone formation?
I have a purified protein and I am wondering if it is possible for the protein to migrate from top chamber to lower chamber in the absence of any cells using the Boyden chamber assay. I've...
15 May 2024 4,380 0 View
I am trying to purify a cell lysate using a 1 mL His-trap column but the viscosity of the lysate is clogging up the column. What are some solutions to this?
21 February 2024 371 3 View
What's the longest time recommended for perfusing a heart on the Langendorff?
13 November 2023 4,475 2 View
Good afternoon all. If someone would kindly answer the following questions, it would be greatly appreciated. Suppose I have a function f[x_,y_] := x2 + y2 + xy2...
02 October 2023 7,611 2 View
This is an SDS-PAGE silver stained gel. The sample is from an FPLC fractions mixed with sample buffer (has SDS and DTT). I'm not sure why the lane has darker edges all the way through...
23 August 2023 2,636 2 View
Colt J, Orwicz K and Bouck G 1991 Water quality considerations and criteria for high-densityfish culture with supplemental oxygen Proceedings of Fisheries Bioengineering Symposium:American...
23 August 2023 1,729 1 View
I am aware that i can do that by simply multiplying it by the norm, but some reflectance are above 100 hence, the norm equation would not be applicable. So, I measured the absolute reflection of a...
21 July 2023 6,738 1 View
I have fractions collected of my protein from FPLC (liquid chromatography) and I am wondering how I am best able to load my samples on a SDS-PAGE gel after lyophilizing/freeze drying the...
22 March 2023 4,020 4 View
I am using Alphafold for protein modelling using this link: https://colab.research.google.com/github/sokrypton/ColabFold/blob/main/AlphaFold2.ipynb recently, it gives me the output as PNG, JSON...
07 February 2023 778 4 View
In a first part, I started the isolation of strains of actinobacteria from agricultural soil where pesticides are used intensively in order to obtain isolates capable of degrading them but my...
21 November 2022 4,642 4 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...
15 July 2024 4,615 1 View
Is there any benefit in knocking out the interferon receptor in 293T cells? In the context of creating RNA viruses 📷
13 July 2024 3,382 0 View
The column is used in Ion Exchange Chromatography of Monoclonal antibodies and the method of analysis is based on pH gradint.
13 July 2024 7,462 1 View
Regeneration Studies for the PVDF membrane and PVDF/CuO nanocomposite membrane were carried out by chemical cleaning.
07 July 2024 7,033 0 View
Hi, I'm working on heart flow cytometry, and I use anti-mouse CCR2-BV785 (biolegend, cat150621) for staining, 20 minutes, 4 degree. But I couldn't see any CCR2+ cells. So I'm not sure if it's...
03 July 2024 3,969 4 View
I seek to understand the theoretical basis for selecting the optimal temperature for the thermal regeneration process of exhausted adsorbents, such as activated carbon loaded with dye molecules....
28 June 2024 1,867 3 View
Hi, I am trying to measure the area of the caudal fin in zebrafish, to study regeneration. I need to measure the area of the regenerating fin every 3 days after injury. So far, i have been using...
27 June 2024 5,068 1 View
Hi all, Second question on the same or so topic. I am working on subroutines (UMAT and others) for Abaqus to implement Tsai-Wu failure criteria for non-composite structures (I work on long...
25 June 2024 2,955 1 View
I need to set a protocol to culture the bone marrow cell. I appreciate it if anyone help me.
22 June 2024 7,562 2 View