Are there internal modifications (e.g. C-spacers) in DNA template strands that will efficiently block transcription by DNA polymerases?

29 January 2022 0 7K Report

Is it possible to block transcription by DNA polymerases (e.g. Klenow, T4 DNA pol.) by designing a DNA template containing modifications (e.g. spacers, bulky groups) that DNA polymerases are unable to extend over?

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