Vincent Geyer

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Questions related from Vincent Geyer

How can I blunt RNA/DNA hybrids following reverse transcription?

I want to remove non-templated nucleotides that are added to the 3' end of my cDNA by reverse transcriptases, creating blunt-ended RNA/DNA hybrids. Standard blunting protocols (T4 DNA pol./Klenow...

02 March 2023 5,121 0 View

Are there internal modifications (e.g. C-spacers) in DNA template strands that will efficiently block transcription by DNA polymerases?

Is it possible to block transcription by DNA polymerases (e.g. Klenow, T4 DNA pol.) by designing a DNA template containing modifications (e.g. spacers, bulky groups) that DNA polymerases are...

29 January 2022 7,128 0 View