17 August 2016 1 603 Report

My classmates and I plan on conducting a study about the different epiphytes found on seagrasses here in the Philippines. Do you guys know of any reference we can use for ID and characterization? 

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BD CBA cytokine kit assay use with BDFACSDiva and Flowjo?

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What is the best way to convert Multiband sequence DICOMs into Nifti?

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When performing multicolor flow cytometry can one use several different color panels in one experiment?

So let's say I have 3 samples and I am interested in testing different fluorophore-conjugated antibodies against certain antigens by using 12 different colors. Howeveer, aach sample only has 10...

05 April 2018 4,083 2 View

Why do I get fluctuating peak areas?

I carried out an experiment aimed at confirming the presence of aspirin and to quantify the abundance of asprin using standard addition. And using HPLC instrumentation. From the result, the...

05 April 2018 5,916 6 View

ELISA assays: Negative results for known positives?

I am testing insoluble homogenates diluted to 0.25M guanidine HCL. Standards for every kit tested work beautifully (standards also in guanidine HCL dilution. The samples are from human gene KI...

22 January 2018 8,822 2 View

Is there a formula in computing the retention factor values of lichen substances for the solvent dichloromethane?

I am currently working on the identification of lichen substances (secondary metabolites) of manglicolous lichens and I only used thin layer chromatography. The solvent I used is dichloromethane....

06 October 2017 7,939 1 View

When staining targets from 2 different species for flow cytometry, can you use 2 different primary Abs against different species in the same sample?

(if you staining for a mouse target and human target within one mouse (ex, chimera), can this be stained in the same tissue sample? Or should samples be stained separately?)

09 August 2017 7,149 2 View

Can anyone explain why I get a lower effect estimate using two-stage logistic analyses compared to straightforward logistic regression?

I am using a two-stage approach (whereby admissions to hospital are nested within patients) to account for multiple admissions of individual patients during a study period. Intuitively it makes...

21 September 2016 8,379 4 View

Can anyone recommend a good DNA ladder that can be used for products from 100bp to 20kb?

I am looking for an easy to read ladder especially on the upper end. I have a ladder that currently goes up to 12kb but after 5kb the bands are hard to decipher.

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