I have a custom made siRNA to Cx36 mRNA and not getting enough knockdown (~10-20%) in the protein expression when I use the native siRNA in the medium of my retina tissue. So far we haven't used any lipofection, but I'm sure (according to the literature) you can make the cationic/native lipid mixture yourself and it 'll work. Any suggestions for a protocol?
Dear Tamas.....
Hope the following article will help you
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2634981/?report=reader
Hi,
you may try Viromers (from Lipocalyx). I am working with Viromers too and I am very satisfied with transfection efficiency. Viromers are synthetic polymers and zero in charge. Due to their endosome escape mechanism I get higher transfection efficiency compared to standard transfection reagents.
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