Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Do Fire damages Concrete Structures Below soil?

Hi All, I am working on the rehabilitation of a fire-damaged structure. Should I consider the Column below Soil structurally deficient. visually it doesn't look like fire affected the column...

12 January 2021 5,401 3 View

Are there any Uni-Dimensional scales available for social media motivation?? Or simply motivations to use social media in context of luxury brand?

I'm looking for Uni-dimensional scales for measuring motivation towards using social media. Or simply social media motivation. In context of social media marketing and Luxury fashion Brand's brand...

13 December 2020 4,929 3 View

Are there any Uni-Dimensional item Scales available for Social Media Marketing? kindly recommend me articles or item scales for Uni-dimensional SSM ?

I couldn't find Social media marketing uni dimensional item scales. Kindly recommend or share with me articles having only uni dimensional measurement scales of Social media marketing to check its...

09 November 2020 8,793 3 View

How do I make 1ug/mL of a stock solution from 10mg/mL of a mother solution?

I have a liquid compound. The volume is 100uL. On the vial of this solution is mentioned 1mg. On the data-sheet is mentioned 10mg/mL. I want to make 1ug/mL of stock solution in ethanol. How much...

21 September 2020 4,815 1 View

Can all network UE decode the information transmitted by a eNB through Physical Downlink Control Channel (PDCCH)?

A wireless network consisting of two UEs (A & B) and an eNB. The eNB transmits control information to UE A through PDCCH. Can UE B also decode this information? Or is it permissible for UE B...

07 August 2020 2,628 2 View

What is the Distance Distribution of random point in a clustered environment?

How to find the distance distribution of a random point in a cluster from the origin? I have uniformly distributed cluster heads following the Poisson point process and users are deployed around...

04 March 2020 6,134 3 View

Minimum ITS sequence length required to accurately identify the Genus in microalgal species?

The microalgal ITS region has about 600 base pairs in total. Are 400 bases enough to identify the genus of the organism? Can it be used to hint at the specie too? I am getting 97% query cover and...

18 January 2020 2,357 3 View

Is hardware support for Dsp C6000 available in MATLAB/SIMULINK ?

I'm currently working on a lab project of DSP and I need to connect Matlab with DSP 6713 kit. But hardware support for c6000 in Matlab's latest versions become obsolete now. and when I try it in...

16 December 2019 866 4 View

How do we differentiate between an organometallic compound and an MOF?

How can an XRD or an FTIR Pattern help to identify their nature if it has not been reported previously?

04 December 2019 3,831 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Is there research limitation on the relationships between digital capabilities, market orientation, brand perception and business performance?

I am looking for supporting studies discussing literature limitations on the relationships between digital capabilities, market orientation, brand perception and business performance. Also, a...

14 February 2021 7,457 5 View

What could be an interesting topic for a bachelor's thesis in a finance/marketing/computer science field?

Hi everyone! I would like to write my bachelor's thesis on a topic that's currently relevant in the sphere of finance, marketing or computer science (or if it's possible a topic concerning all the...

08 February 2021 1,300 12 View

Which pump is used to transfer low melting temperature liquid metal from one container to another?

I want to transfer GaInSn alloy from one container to another with the help of an electromagnetic pump. Can anybody suggest me which brand or which company supplies these pumps?

25 January 2021 9,481 2 View

Help me conduct a neuroscience experiment for my master's thesis?

22 January 2021 9,994 5 View

Anyone knows a Postdoc position in Marketing?

Dear Profs, Associate Profs, and Assistant Profs, I graduated with a Ph.D. in Marketing from Graduate School of Business, Universiti Kebangsaan Malaysia (GSB-UKM) in 2019. GSB-UKM is an...

23 December 2020 8,873 3 View

Are there any Uni-Dimensional scales available for social media motivation?? Or simply motivations to use social media in context of luxury brand?

I'm looking for Uni-dimensional scales for measuring motivation towards using social media. Or simply social media motivation. In context of social media marketing and Luxury fashion Brand's brand...

13 December 2020 4,929 3 View

Which countries have comparatively more secure future, agricultural or industrial?

Agriculture provides for almost all the basic needs essential for survival and industries provide mostly the luxury commodities required for comfortable life. However, some countries are heavily...

07 December 2020 2,153 15 View

How to know if 87Sr/86Sr is reliable for Sr isotope chemostratigraphy in marbles?

I am currently looking for parameters to know if 87Sr/86Sr data is reliable for chemostratigrahy in marbles, and/or parameters to know if those marbles have had some post-depositional...

30 November 2020 9,032 8 View

Have you tried the Tissue Total RNA Mini Kit, with DNase (50) rxns from Geneaid?

We are going to work with very small tissue samples and we are looking into different kit from many brands and this one is significantly less expensive, but we haven't heard about it. Has anybody?...

18 November 2020 1,387 1 View