I mostly read some colorimetric methods. Are there better ways to quantify them, especially when carbohydrates are not the core of the study but needed just to be able to calculate their percentage compared to other nutrients in a certain green leaf material?
Total carbohydrates can be calculated simply by subtracting other chemical constituents by the following formula.
T-CHO (% DM)=100-Cude Protein (% DM)-Ether Extract (% DM)-Ash (% DM)
This is useful for most of food and feed ingredients, hopefully, leaves of your interest too.
An attachment is for more confirmation.
Article Nutrient utilisation and methane emissions in Sahiwal calves...
If you do not want to determine Carbohydrate by difference... check this FAO link out that lists some options.
http://www.fao.org/docrep/w8079e/w8079e07.htm
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