Although, silver staining is the best option to visualize SSR alleles in Poly Acrylamide gel electrophoresis, it comes with number of limitations. The main thing is, you cannot reuse the gels. There are some good alternatives, but it depends on the type of reader or gel imagining devise you have,

1. Mix EtBr at the lower buffer tank and visualize in UV light.

2. IR based fluorescent dyes are always the best option and scan in appropriate scanner.

Ramesh

its depend on your product size and quantity . if your product is more than 20 bp and your product quantity more than 3 ng per band . in this case you can use ethidiom promid or sibergreen

Thanks for your answers. If I want to use sibergreen or ethidiom promid , how should I use them. How does the protocol change? Do both of them visualize under UV? thanks again

Yes, both can be viewed in UV light. Protocol is same for both, however, follow manufactures instructions.

Mixing with EtBr and visualize under UV light is a good choice.

Make a bath of EtBr and put directly your gels insides and than visualized your gels under UV

You have to make agarose gel with high concentration and Mixing with EtBr and visualize under UV light unitil yoh can see band heterozygote, albet I suggest for you use achryle amid gel with silver staining

Try EtBr staining for 5 to 10 min under dark condition ,then wash with distill water for 1 min and obesreved it under UV.