Any way to separate out fluorescent channels from a composite image?

Hi there, I've been using the EVOS Auto II microscope for some time now and usually I will save the transmitted light/bright field image and the images from fluorescent channels as separate...

15 February 2021 546 3 View

Cells have high and low confluency in same flask?

I'm currently growing OVCAR3 and OVCAR8 cells in T225 flasks. I returned to the lab a couple of months ago after being away for nearly half a year due to COVID-19, and since I've returned I've had...

23 October 2020 3,683 4 View

How do I perform procrustes analysis after Bayesian PCA?

I'm testing different methods of calculating missing landmarks for geomorphic morphometric analysis of rodent footprints. After an initial literature review, I came across Bayesian PCA (BPCA) and...

02 March 2020 6,756 3 View

CD45 stain disappearing after intracellular staining (image cytometry)?

I'm having issues with the stability of my CD45 stain conjugated with APC. I'm staining PBMCs for Circulating tumour cells. If I do not do the intraceulluar (CK) staining and simply CD45 stain...

02 January 2020 9,609 2 View

Tips for extracting DNA from clotted, frozen avian red blood cells?

I have clotted, frozen avian red blood cells that I am attempting to extract DNA from (for eventual micro satellite sequencing). I am using the Qiagen DNeasy Blood and Tissue Kit. The protocol was...

18 March 2019 8,656 3 View

What's the best way to contact you?

I'm working with some folks involved in the Herstory Project - I think they would be interested. [email protected] Thanks.

09 December 2018 3,461 3 View

I'm working with some folks involved in the Herstory Project - I think they would be interested. What's the best way to contact you?

[email protected] Thanks.

09 December 2018 4,784 2 View

Does anyone know this cell phenotype?

25 July 2018 744 1 View

Isolating tumor RNA in a sterile manner

Has anyone isolated tumor RNA from cell lines for use in cell culture? In all the papers I've read I can see no mention of isolating the RNA in any more sterile manner than is required for any RNA...

20 February 2017 8,530 3 View

Triple transfection mechanism : does GFP testifies of efficient transfection when expressed ?

Hi everyone, Pardon me if the question sounds a bit stupid but I just would like to be sure to understand the triple transfection mechanism. I'm performing triple transfection for AAV2 production...

23 September 2015 2,726 12 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Do Fire damages Concrete Structures Below soil?

Hi All, I am working on the rehabilitation of a fire-damaged structure. Should I consider the Column below Soil structurally deficient. visually it doesn't look like fire affected the column...

12 January 2021 5,401 3 View

How do i handle Ti and/or Aluminium powder. Since in the instruction on the container is written that CATCHES FIRE SPONTANEOUSLY IF EXPOSED TO AIR ?

i am working on max phase preparation......i am a newly joined PhD scholar. I wanted to make MAX phase of Ti3AlC2 , in most of the literature they are using the metal powder of Ti and Al for the...

23 December 2020 7,122 8 View

Dear researchers, anybody can suggest some classification algorithms for fire images?

dear researchers at present we are working in fire detection projcts ,i have a plan to implement the CNN with SVM for classification method. if you hav any best or recent techniques please let me...

01 November 2020 4,142 7 View

How can I track the decrease in initiation events of combustion?

Hello, I am interested in tracking the decrease in initiation events of different wildland fuel combustion. Apart from the experimental procedures, is there any simulation software that can help...

12 October 2020 7,905 1 View

How to calculate total fire counts by using MODIS/Aqua Thermal Anomalies/Fire 5-Minute L2 Swath 1 km dataset in MATLAB?

I want to plot spatial variation of monthly averaged total fire counts for my region of interest. I am using MODIS/Aqua Thermal Anomalies/Fire 5-Minute L2 Swath 1 km data which is in hdf format...

12 October 2020 6,477 3 View

How to heat a reactor up to 900C?

I'm taking a bachelor design, I'm supposed to maintain an endothermic reactor at 900C and 30 bar. The typical jacketed reactor is not really feasible and I'm thinking of fired heater to maintain...

07 October 2020 8,558 5 View

Curd intake advisories in Traditional System of Medicine?

Ayurveda, TM of India, advises to avoid Curd intake in diseases that have Kapha dosha predominance like Diabetes, Respiratory problems; in diseases arising out of Raktha dusti (Blood tissue...

03 October 2020 780 1 View

Can COMSOL be used for (wild)fire modeling?

Has anyone any experience about fire modeling with COMSOL multi-physics? The software can simulate jet flames and combustion; however, as far as I have checked the literature, very few surveys...

30 September 2020 4,111 1 View

How can I filter MODIS active fire data to exclude single fire burning several days?

I am using MODIS MCD14DL active fire data (https://firms.modaps.eosdis.nasa.gov/download/)to monitor fire distribution in my area of interest. I would like to exclude for each selected hotspot...

29 August 2020 7,952 5 View