The undersigned is in the process of purification of immunogenic proteins from the cancer tissue. Total protein available with us is partially purified. The immunogenic band is resolved at the top cathodic region by native PAGE resolution. A facility of Prep cell 490/491(M/s Biorad)/ Protein Purification System (M/s ATTO) will suffice for this separation. Could anyone tell where such facilities are available for preparative PAGE isolation of immunogenic protein bands?
Alternatively, I would also like to get some more ideas how can I separate this protein band resolved at the top cathodic region by native PAGE resolution?