Are there any differences in the blood brain barrier of C57Bl/6 and balb/c mice?

More Nisha Kuzhuppilly Ramakrishnan's questions See All

How to determine LOD values?

I am performing fluorescence experiments using a ligand to detect metal ions. I want to determine the Lowest Detection Limit (LOD) using the formula LOD = 3σ / K. However, I'm uncertain about...

19 July 2024 1,086 1 View

What sustainable strategies can European luxury brands adopt to effectively expand their market presence in Asia?

I am currently working on my thesis, which focuses on the expansion of European luxury brands into key Asian markets, specifically China, India, South Korea, Thailand, and Japan. The main topic of...

15 July 2024 4,130 0 View

Facing issues when analyzing raw LC-MS/MS data with MS-DIAL?

I am facing a problem with my Waters MSe raw data. I have tried to open my raw data with AIF mode, after converting my file to ABF format for MS-DIAL, but everytime my system crashed. Later I...

14 June 2024 2,492 1 View

For how much time single cell of Xanthomonas oryzae can be alive at room temperature in nutrient deficient condition?

20 May 2024 3,673 1 View

What is the dilution rate? How can it be calculated during semi continuous cultivation?

In a semicontinuous cultivation strategy, how to calculate the dilution rate? Is the dilution rate is same as the renewal rate? What are the parameters required during this cultivation?

17 May 2024 4,291 0 View

Recommended molecular weight of PVP for electrospinning?

Could anyone confirm if Polyvinylpyrrolidone (PVP) K-30 with a molecular weight of 40K is suitable for electrospinning? If not, could you recommend an appropriate molecular weight of PVP for...

05 March 2024 3,066 0 View

Western Blot protocol??

We are running western blot for checking the ag-ab interactions.our proteins are from molecular weight 250 to 7 kda.But we are facing some technical issues with the transfer of proteins(i'm not...

28 November 2023 1,408 2 View

How to extract promoter sequences from genome file using GFF?

I have both the Gene GFF file and the genome file available and would now like to proceed with identifying promoter sequences from my files. However, I am not sure which software is best to use to...

19 October 2023 2,291 2 View

How to stabilize overexpressed Protein?

I have cloned a gene in pET28a vector, and induced it for 5 hrs with 1mM IPTG. The cells were centrifuged from 1 ml culture (Uninduced and induced both), and the pellet was suspended in 200...

25 August 2023 3,448 1 View

Agarose gel electrophoresis?

I did agarose gel electrophoresis with different concentrations of Dna:250ng,500ng and 1 microgram.But the bands were in smear form,from which I concluded that the dna was degraded.But when i...

10 August 2023 6,937 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

04 August 2024 6,282 1 View

Nurul Adhwa Rahman

Hi Nisha. I read about this topic yesterday, however on different strains. You can read the paper in the link below:

Article Strain influences on inflammatory pathway activation, cell i...

Thomas J Raub

Not in our experience testing 1000s of cmpds iv over 20 yrs while considering the 2-3 fold variability in PK data.