Are there any COS-7 cell lines available for purchase that constitutively express any fluorophore-tagged cytoskeleton proteins (such as actin or microtubules)?
Can anyone please share any article showing the minimum volume of sample/ protein concentration to perform a Laser ionization mass spectrometry (LIMS) or any mass spectrometry techniques in general?
12 August 2022 3,361 4 View
Can anyone please suggest a cheap hydrogel brand available to buy for 3D cell culture? Or if someone can share a recipe to prepare homemade hydrogel (for 3D cell culture), that would be fantastic.
13 May 2022 8,369 4 View
I tried to grow monolayer of cells (I use HEK 293) on agarose and acrylamide gels, I am not able to get the monolayer. Can anyone please share a working protocol?
08 April 2022 885 0 View
All Silicones are soluble in water or insoluble!
01 January 1970 3,556 6 View
Are the mother and daughter centrioles perpendicular to each other in all types of cilia basal bodies/ MTOC?
01 January 1970 289 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View