I am looking for some chemical / dye / reagent that can react directly with hydrogen peroxide to produce color, for simple colorimetric detection. Mostly, hydrogen colorimetric assay involved peroxidase enzyme. I am looking for the hydrogen peroxide reaction to produce color without peroxidase enzyme
Thank you
H2O2 oxidizes iodide to triiodide; this in turn can be detected with starch or with leuco crystal violet.
Right. Iodide, iodide anion, reduced indigo are well-known peroxide indicators. You can find an incomplete list of much more of these on Wikipedia https://www.google.hu/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&uact=8&ved=0CCEQFjAA&url=http%3A%2F%2Fen.wikipedia.org%2Fwiki%2FRedox_indicator&ei=ErNIVJXbMaPiywPMpYLgAw&usg=AFQjCNELwmtn3hUAG31JhwU7sHUFuNC93w&sig2=h3ue3Qw3aeiqtGcjVdbNlA so that you can chose the most compatible reagent for your system. There are also commercially available, cheap peroxide strips, which allow you the determination of approximate concentration of H2O2.
Titanium oxalate or ABTS. You can find references to the use of both in the reference.
Article Chemical disinfection of combined sewer overflow waters usin...
Diluted hydrogen peroxide may be used as a standard for absolute
quantitation, provided it is standardized by UV absorption measurement
prior to dilution. Hydrogen peroxide solutions can be unstable,
so standardization is essential for accurate results. To determine the
concentration of a Hydrogen Peroxide solution, measure the absorbance
at 240nm and use a molar extinction coefficient of 43.6M-1cm-1
(JBC 245, pp2409-13, 1970) A standard 3% solution is 0.88M,
and at a 1:100 dilution would have an A240 of 0.388.
https://www.researchgate.net/publication/11817140_Workplace_monitoring_of_hydrogen_peroxide_using_titanyl-coated_sorbents
Dear all,
I am considering to purchase a catalase activity kit to determine the effectiveness of the catalase inhibitor that I am using, 3-AT. After carefully studying the protocol, I have a concern. How does the kit account for the activity of other enzymes that can also neutralize H2O2? GSHPx as well as the thioredoxin system can also neutralize H2O2.
Please share your thoughts, especially if you have experience with catalase activity kits.
MH
I know the answer. You have to visit my lab to have the answer. Seriously.
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