You can use Bouin's solution for fixation of testicular tissue.

Thank you very much for answering. How about for skeletal muscle tissue?

What is your eventual aim for the tissue? We use 4% paraformaldehyde prior to paraffin embedding.

You should get into contact with Basel anatomic institute (human university). They have developed a special solution. However, I do not know, what it is. Anyway, it smells much better than formaline.

I strongly recommend this article:

Human body preservation - old and new techniques.

Brenner E.

J Anat. 2014 Mar;224(3):316-44. doi: 10.1111/joa.12160. Epub 2014 Jan 18.

I made excellent experiences with Peters Solution for gross anatomical specimens.

Dear colleagues,

The Anatomy Department of the Nova Medical School (New University of Lisbon), in Portugal, has been devoting efforts for a few years, on this issue of perfecting embalming techniques with good preservation of tissues, to permit undergraduate students to dissect regularly, even in our warm climate.

Lately, we have achieved such good results with our embalming technique , that we are currently using some of our cadaveric donations for post graduation courses, hands-on surgical specialisation courses, and surgical innovations training.

Our specimens keep a well preserved aspect, both in terms of muscular tissues , as with abdominal and pelvic organs.

I should recommend the reference to an article published by J. Goyri-O'Neill and co-workers, where you'll find photographic evidence of our work, in a full-text available on Research Gate:

https://www.researchgate.net/publication/244479992_Improvement_of_the_embalming_perfusion_method_the_innovation_and_the_results_by_light_and_scanning_electron_microscopy

(Hope this was useful!)

Maria.

Article Improvement of the Embalming Perfusion Method: The Innovatio...

Dear Maria,

Thank you for quoting my work.

In reference to the above issue, I should remark and emphasize a few more topics:

Our 15 years experience with these conservation methods are still under improvement.

In our next work, we will present reference to the experimental use of several embalming techniques, with conservation times between one or one and half year, up to five years.

Our best results were obtained during our first years of experimentation, when we developed our perfusion pump in straight collaboration with CEFITEC and the Department of Physics of the Faculty of Technological Sciences of our University.

The development of this perfusion pump allows complete vascular perfusion in closed circuit with perfect control of debits and capillary perfusion, with pulsed impregnation of tissues, simulating the cardiac rhythms.

The specimens we use, both for undergraduate dissections (over a period of six weeks of manipulations), as for post graduation courses and surgical training sessions, bear great quality and endurance, as you can check from this Prezzi presentation in attachment http://prezi.com/qk-muuogpev7/eaca-lisbon-dissection-room-june2013/?utm_campaign=share&utm_medium=copy

The materials we use for current conservation, consisting of a modified mixture of Theil, are quite inexpensive and easy to purchase, and, most of all, less toxic than Formaldehyde, for the staff. (Should I remind that Formaldehyde is one of the few -9- well-documented cancerigenic materials still in the market)

All the best,

João

Thank you for all the useful information. I am looking to preserve Dead Sea turtles that have been washing up on the beach, for display purposes and some for autopsy. I assume there are other factors to consider in regards to preserving turtles, correct? Any suggestions or ideas would be greatly appreciated.

thnk you,

samuel

Dear Samuel,

Maybe Saturated salt solution method may be useful, because formalin hardens soft tissues

We need to come up with other techniques, like a saturated salt solution, because formalin hardens soft tissues and is also unhealthy.