What should a smart water system look like?

Our team is designing a smart water C&C dashboard for a smart city initiative. As we look into the current smart water system landscape, we couldn't reconcile to a definitive solution that...

16 February 2021 1,094 3 View

Anyone has an experience extracting proteins from rectal swab samples?

I am working with rectal swab samples in the field of colorectal cancer research. Does anyone have experience (or can point towards any relevant literature) of specifically working with proteins...

02 February 2021 3,622 3 View

Is it possible to ligate two restriction ends those have no complementary bases?

In my lab,we are trying to construct a control plasmid. So we have to remove the insert gene which has two restriction ends:one is XhoI and other one is Hind III.So after double digestion we want...

01 January 2021 3,271 4 View

How to generate topology file for ligand for Gromacs?

Dear all, I converted a PDB file into Mol2 file using Openbabel (H added) and then fixed the bond order number using this command: perl sort_mol2_bonds.pl jz4.mol2 jz4_fix.mol2 When I try to...

22 December 2020 303 4 View

Can we used PTOTACs to improves the RNA vaccines immunity period ?

Using targeted degradation of non structural proteins of viral capsid can we lengthen the vaccines effectiveness

21 December 2020 7,200 1 View

Can I reuse the NAP-5 colums(GE)?

Hi all, I am using NAP-5 disposable columns from GE for removing DTT from my protein. Can I use the same column again for the purpose of removing DTT. Can you please mention the protocol for...

10 December 2020 3,199 5 View

How to reduce (which reducing agent) a disulfide bridge in a protein and how long does it remain reduced?

I want to reduce the disulfide bridge in my 7kDa protein. My protein has only 1 cysteine at the surface and thus makes a homodimer using a disulfide bridge. I want to reduce the disulfide bridge...

24 November 2020 3,211 5 View

Is it possible to get higher order of diffraction (n>1) in HRTEM?

The SAED pattern, we obtained from HRTEM shows the interplanar spacing (d) that corresponds to a higher 2theta value than the Powder crystal X-ray diffraction pattern for the same organic...

02 November 2020 8,073 3 View

What are the efficient methods available to test the soil fertility?

Want to understand the scientific mechanisms to know type of soil suitable for agricultural purpose.

22 October 2020 4,941 3 View

How to capture UV- fluorescence from a substance using a camera?

I have a liquid that glows when UV light is shined. However, when I use my phone camera I can't see the glow, I just see a violet hue. What is the best way of capturing the fluorescence-glow from...

02 October 2020 8,664 3 View

Cells not growing in large scale protein expression?

I am using KRX cell. I have grown my overnight cultures at 37 degrees. When I inoculate 1L TB media, my cells do not grow. I have also tried using 25 degree incubation of overnight cultures and...

10 February 2021 3,871 3 View

What is the best way and technique for serial dilution of bactria to get accurate number of colony ?

Hello I am infecting cells with bacteria in a well and then I am doing serial dilution of this well 1 in 10 and culture the two last dilution on MHA agar I have difficulty in getting accurate...

26 January 2021 9,256 5 View

How to lessen cell growth?

I am working on the 4T1 murine breast cancer cell line. from the first day I bought this cell line, the cell's growth rate is extremely high. I intend to make these cells resistant to a single...

24 January 2021 2,884 3 View

How to calculate the CFU/ml for Mycobacterium tuberculosis growing in 7H9 broth?

I am running an experiment in which I need to calculate the CFU/ml of a Mycobacterium tuberculosis growing in 7H9 broth. Is there a reliable method to calculate cfu/ml for MTB since a culture of...

22 January 2021 10,027 2 View

How to revive bacteria from glycerol stock?

19 January 2021 7,025 4 View

How long I can store plasma in -80 oC before doing Growth differentiation factor-15 assay ? , Is it different when I stored plasma at -20 or -70 ?

I want to do GDF-15 assay on multidrug resistant tuberculosis patient

29 December 2020 6,764 1 View

What are the invivo screening methods for anti TB drug?

what are the invivo screening methods for anti TB drug?

25 December 2020 722 1 View

Biotech final year project for a bioinformatics beginner?

So my last year project is Drug Efflux Pumps and Persistence in Methicillin Resistant Staphylococcus aureus and we gonna focus on persister cells to study the path way of antimicrobial...

09 December 2020 2,574 2 View

Iam looking for articles on mycobacterium tuberculosis?

Hellow my fellows Iam working on Real time PCR technique for detection the gene who is responsiable on Tuberculosis as well as the AFB stain so i hope that any one have worked on the same project...

02 December 2020 2,123 3 View

What is a typical DBC of an affinity column?

I'm purifying TB antibody and the columns we are using only seem to have a DBC of about 0.15 mg/ml. This seems very low to me, but I am a protein purification novice. Is this normal?

14 July 2020 1,443 4 View