Are there any alkaline yeast culture media?

More Hong-Guang Zha's questions See All

I cultured microorganisms obtained from the soil on a medium. Can you identify what kind of microorganisms these are?

I cultured microorganisms obtained from the soil on a medium. Can you identify what kind of microorganisms these are? Is it possible that this microorganism is Stenotrophomonas panacihumi?

22 May 2024 875 4 View

What are the statistical approach for servant leadership in F&B?

To have multiple case study exploration among Malaysia restaurants that study the impact of servant leadership, what are the best statistical approaches for the case studies?

01 March 2024 2,737 1 View

Do solar winds impact the spread of communicable disease on the earth?

We know that solar winds had a 11 year circle and also human respiratory diseases also outbroke every 11-12 year. Is there ant connection between solar wind's strength and the pandemic...

18 February 2024 6,280 5 View

In low doping level, i want to know why N-type threshold voltage has negative charge?

Basically, we know that the N-channel MOSFET has a (+) threshold voltage. However, the graph has a value of (-) depending on the acceptor doping concentration in the substrate and oxide capture...

24 January 2024 7,817 0 View

How to calculate the osmolality of Hepes solution?

Here is an excerpt from Kollmann et al. (2020, J. Physiol.): "This ring was placed in a recording chamber continuously perfused with 37°C aerated Hepes solution containing (in mM) 136 NaCl, 10...

09 January 2024 4,766 2 View

Heme in protein - MD simulation?

Dear all, I am currently working on a protein simulation with a heme group in the protein. I am wondering whether anyone can tell me how I can set it up in GROMCAS with charmm forcefile, since...

17 December 2023 4,392 2 View

Is it possible to use SPSS PROCESS MACRO(Model 1) to test moderation with categorical IV + binary DV + continuous Moderator?

IV: categorical - (5 categories with 1 and 5 being the extreme indicators) DV: binary - (dummy coding has been done.) Mo: continuous 1) Is it possible to use SPSS PROCESS MACRO for moderation...

08 November 2023 4,421 1 View

Western blot sample prep problem?

Hello, I have a question about western blot sample prep. First, I am using adherent cells and RIPA buffer. After centrifuge at 4 degrees Celsius, small pieces are still found when collecting the...

01 November 2023 7,135 0 View

Does the small intestinal manometry measure the gas pressure or liquid pressure?

Small intestinal pressure is one of the GI motility indicators and its manometry is very advanced nowadays. I wonder if there is any liquid inside the lumen during manometry. I already know the...

28 October 2023 2,491 1 View

How to combine trajectory in GROMACS when I repeat the same simulation for three times?

Hi all, I am wondering how I can combine the trajectory in GROMACS. I used the coding 'gmx trjcat -s xx.tpr -f xx-1.xtc xx-2.xtc xx-3.xtc -o xxx-combined.xtc' and 'gmx trjcat -s xx.tpr -f...

16 September 2023 2,539 0 View

What is the reason for current dropping in OER , LSV curve?

I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...

10 August 2024 3,629 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Do you know of any online international conferences that offer free discussions?

Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.

28 July 2024 6,501 0 View

What exactly is it about biotechnology and medical research?

I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together

28 July 2024 3,798 4 View

Dominique Liger

Hi there,

What kind of yeast are you expecting to grow at pH11?

Hong-Guang Zha

Thanks for your question. In fact, I am not quite sure whether ant yeast or mold living in the sample I am studying on whose pH is above 10. I want to prepare some alkaline medium and try to isolate them. Maybe there is no yeast in such high pH surroundings, I want to test it.

OK, so I guess just adding a buffering system to yeast media should be OK (HCO3Na/NaOH for instance)... But i don't think you will isolate any yeast from this media.

thanks for your suggestion. I will do try it.