Are the MW protein markers in precision all blue strep tagged?

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Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

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Simone Morra

Hi there, mine do the same. Many MW from Thermo or Bio-rad are Strep-tagged.

Angela T Harrison

To my knowledge the Biorad unstained will react with streptactin but not the all blue. We use the unstained markers for western blotting analysis with an HRP tag.

Maria C Davis

Thank you both!

Viktor Y Butnev

Only Bio-Rad Western C MW markers react with Streptactin.

Precision All Blue MW markers should not be recognized by Streptactin. Probably, your primary (or secondary) antibodies are used at too high concentration.