Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

What are the surgery journal Q1orQ2 indexed in Web of science and are fair in publishing manuscript?

Rapid publication journal

31 December 2020 4,774 3 View

Annealing temperature in RT PCR - Positive control differes from target gene?

The primer annealing temperature , after the gradient PCR analysis, for the target gene differs from the optimal annealing temperature in housekeeping gene (positive control). What to do ? The...

02 July 2019 810 3 View

What statistical test can i use on my data?

I am researching the relationship between animal biodiversity and physics-chemical properties of a clean and polluted burrow dyke. I have the species abundance for both burrow dykes, as well as...

26 June 2019 569 2 View

Segregation coefficient of self-compacting concrete and small superplasticizer overlaps?

What do you think how important, for segregation coefficient of SCC, could be very small superplasticizer overlaps during the process of making SCC? I made some observations in my work: Some...

04 May 2019 6,951 4 View

What policies may bring about sustainable reform and development?

Social disparities rise amid volatile economic and political times today. What may be constructive efforts in policy form to start resolving issues that cause such state of affairs?

16 March 2019 1,086 4 View

How can I improved the power (accuracy) of my Deep Learning Model?

Hey everyone, me and my colleagues are programming a Deep Learning Model with tensorflow. Our goal is it to predict chemical toxicity. Our input are chemical drugs in SMILE-Strucuture. So, how...

07 June 2018 7,920 3 View

What are uses of waste in geotechnical engineering?

waste use in improving soil engineering specification such as disaster waste, cement waste and others

02 August 2017 8,663 16 View

Dummy/ real data Request

02 July 2017 5,438 2 View

Can any body kindly share questionnaire on "Productivity of employees". ?

11 November 2016 9,427 6 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View