For defibrination, blood was collected in a sterile bottle containing small glass beads and with constant manual agitation during the bleeding process. For citrated blood, blood was collected in a standard tubes for human blood sampling but in exactly the right ratio of blood and anticoagulants (1,4 ml citrate : 10ml of the blood). In both cases, blood is liquid and can be used to make microbiological media (blood agar).

Details about the procedure and diagnostic accuracy can be found in the published work of the authors on the link: http://www.plosone.org/article/info:doi/10.1371/journal.pone.0006141

Both defibrination and citrination can be done to stop blood from clotting/coagulating. However, the methods are different; one stops coagulation by removing the fibrin/fibrinogen from the blood and the other 'messing up' the actions of calcium in the clotting cascade.

For defibrination, blood was collected in a sterile bottle containing small glass beads and with constant manual agitation during the bleeding process. For citrated blood, blood was collected in a standard tubes for human blood sampling but in exactly the right ratio of blood and anticoagulants (1,4 ml citrate : 10ml of the blood). In both cases, blood is liquid and can be used to make microbiological media (blood agar).

Details about the procedure and diagnostic accuracy can be found in the published work of the authors on the link: http://www.plosone.org/article/info:doi/10.1371/journal.pone.0006141

Thank you Jasna fo your clarification and for the paper. it was very helpful for me.