How to Detect Water in THF (Tetrahydrofuran) ?

Hello, we have solvent purifier system in our lab. But There is some unwanted results for our experiment. We are suspecting purity of solvents. Are there any simple methods to detect water in THF?

19 November 2020 114 5 View

If an animal feeds mainly on algae but also eats cyanobacteria and insects, which group does this animal belong to; herbivore or omnivore?

I wonder if a fish (Random sp.) feeds mostly on plankton but also consumes cyanobacteria and some insects (Chironomidae, Simuliidae) at much lower rates, can we classify them as omnivore?

03 March 2020 8,177 4 View

How does the amount of amino acid exceed the amount of crude protein in fish meal (Dry matter basis)?

I read a SCI article where the author analysed several fish meals' amino acid profiles and crude protein levels. I.E., The findings indicate that the crude protein level is 79.50g/100g for fish...

16 July 2019 2,039 6 View

Which one should I choose from the results of a sample with different amino acid analysis methods?

I have a protein source with varying results of amino acid profile. For instance; when the sample is hydrolysed with "6 M HCI" L-Valine level is 0.24g/100g whereas it is 3.08g/100g when...

23 February 2018 5,648 2 View

What is the difference between higher order (second order) confirmatory factor analysis and bifactor analysis?

24 July 2017 5,178 5 View

How to determine total RNA concentration when you have large amount of miRNA?

I am isolating total RNA with miRNeasy micro (PBMCs) miRNeasy serum plasma (serum) and exoRNeasy (serum/exosome) for Next Gen Sequencing for miRNA. I believe my final product should contain mostly...

30 March 2016 3,006 4 View

How can I do a reaction between polyamine and lipoic acid NHS ester in an organic solvent?

I want to do this reaction but as far as learned from literature, NHS ester-amine reaction is done in a phosphate buffer solution between pH 8 to 9. However, lipoic acid modified polymer...

27 June 2015 1,731 3 View

An economic method for the fluorescent labeling of PCR fragments?

I have a trouble with my pcr reactions and i want to ask you where is my fault.I designed six primer pairs according to Schuelke's M13 methods but I did not get any reaction.One of my primers...

23 December 2014 4,623 5 View

What is the reason for different fold changes in gene expression from independent experimental repeats in Real-time PCR experiments ?

25 October 2014 7,228 3 View

How should I calculate a touchdown PCR reaction with a low dna quantity?

14 September 2014 2,091 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View