I have been troubleshooting these IPSC KOLF2-C1 cell lines. Attached is an image that shows the IPSC after passaging them. They look spontaneously differentiated and have a shining blob on top of each IPSC colony. Media is clear and not turbid. 1% Penicillin/Streptomycin is added. Are these cells contaminated?

The protocol for passaging is as follows:

- 6-well plates are coated with Geltrex diluted in DMEM/F12 at 1:100 dilution. Incubated in 37C incubator for at least 1hr.

- Remove media from wells and wash with HBSS. Add Versene to each well and incubate for 4-8mins in incubator.

- Geltrex is removed and StemFlex media is added to each well and placed in incubator.

- When cells are loose, EDTA is removed and StemFlex media is added to each well and gently dislodged from plate.

- Cell suspension with Stemflex is added at appropriate cell density to each well.

- Media changed every alternate day and passaged every 7 days (They grow slower than expected).