Are LDLR VLDLR double knockout mice lethal?

More Florian L Muller's questions See All

Why does my IRC calculation generates higher energy structure around TS ?

While computing the mechanism of a trimethylsilylcyanation related to this work (10.1021/acs.organomet.1c00070) I managed to optimize the geometry of both reactants and product and locate a good...

26 May 2024 9,925 0 View

How to lyse nuclei without detergent?

Hi, I want to isolate nuclear proteins from the diatom P. tricornutum. I can successfully isolate the nuclei, and can image them under the microscope. But I fail to lyse the nuclei. As they were...

14 December 2023 3,640 1 View

Does anyone know suppliers of high molecular weight PS?

I am looking for high molecular weight polystyrene for technical applications. The molecular weight should be at least 500.000 g/mol or higher. The PDI is not that important. Can anyone give me...

29 August 2023 7,516 2 View

Homemade MDA-Assay protocol?

Dear all, Our group has tried for several months to establish a homemade protocol for MDA-assay (Malondialdehyde Colorimetric Assay) to measure lipid peroxidation levels in cell culture. So far,...

29 August 2023 7,697 3 View

Can somebody help with IDing this salp?

Dear all, I am curious to learn about the identification of the pictured salp individuum. The pictures were taken in October 2022 near San Antonio del Sur (southern Cuba) not far away from shore....

15 August 2023 9,008 0 View

Is there an analytic solution for calculating sag and tension of suspended fiber optic cables in inclined span?

According to a paper published in 50th IWCS conference "Sag-Tension Calculations for Aerial Cable with Linear or Nonlinear Tensile Properties" by T. C. Chu, there are simple equations with one...

06 August 2023 5,991 0 View

How to tespond to a request???

I received a text request from Kim Lawson about ketamine in fibromyalgia. I do not find how to send as I do not have Twitter, nor Kim's email

18 July 2023 4,146 1 View

Simulation Of Pump Down Graph with COMSOL 6.0?

hi community, I would like simulate the pump down graph of my vacuum pump. My first test is to use laminar flow, create an air cube of 1m^3 and add a vacuum pump on a side. The specificity of the...

13 June 2023 8,974 1 View

Which mEOS version to use for PALM?

Hi, I would like to use mEOS to tag proteins to evaluate their subcellular localization. I am new to PALM, and am a bit confused with all the different versions of mEOS available... mEOS2? mEOS4?...

19 April 2023 7,456 1 View

Question to all zooplankton and mollusc researchers: Can somebody help with IDing this small heteropod?

Dear colleagues, On an expedition in the southern Sargasso Sea, subtropical Northwest Atlantic, I encountered repeatedly specimens of the photographed heteropod, never (so far) being bigger than...

28 March 2023 3,057 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View