Does soft algniate hydrogel influence calcium concentration of a solution (cell culture medium)?

05 January 2021 6,066 4 View

Why does a magnetic mirror acting on ballistic electrons not violate the 2nd law of thermodynamics?

Magnetic mirrors are well known in plasma physics. In order to work, the mean free path of the charge carriers has to be at least as long as the helical paths under the influence of the B field....

24 October 2020 8,124 3 View

Collagen Type I Hydrogel dissociates/degradates/desintegrates in culture with cells. Where am I going wrong?

Hi all, I am having trouble with collagen type I as a scaffold for cells. I have a 3mg/mL collagen I solution with pH = 2. I am neutralizing with 1 M NaOH to pH ~7, then mixing with ice cold...

22 October 2020 8,429 3 View

Magnetic monopole pairs generated by photon/photon collisions?

The transformation of two "colliding" photons into a pair of particle / anti-particle was experimentally observed already. But the observed particles are always electrically charged. 1. If such a...

30 July 2019 1,569 18 View

Concentration of TCEP for RNA Isolation?

Hi all, in our lab we would like to switch from Mercaptoethanol to TCEP as a reducing agent for RNA protection during isolation. What concentration do you use? I only found a protocoll from...

01 March 2017 4,234 2 View

Does PDMS cure when it is in contact to water?

I want to seal a tissue/glass interface. The tissue should not be out of the water (buffer) for too long, so I am wondering if I could apply PDMS in liquid form and let it cure under water?...

17 October 2016 3,563 7 View

Which optical E-field sensor would you recommend for time-domain measurements?

For some years, optical E-field sensors (plus remote units) are commercially available; main advantage: the field is much less disturbed than by electrically conductive sensors. We would like to...

21 April 2016 3,688 5 View

Should we use 5-pt or 7-pt Likert scales? Any impact on significance?

Hello everyone A student of mine recently asked if it matters for significance testing whether a 5-pt or a 7-pt Likert scale is used. That is, are effects more likely to become statistically...

13 April 2015 5,188 9 View

Volition in music - Seeking for international colleagues for further development of a volitional online test - interested?

The "will test in music" is available as volitional test for German speaking music students. This test should be provided in different language versions. In a first step, I would like to make an...

27 December 2014 1,946 10 View

Can anybody recommend a good software package for analyzing multilevel SEM?

I need to test an SEM model that involves multiple levels of analysis (individual, group, organization) and I am currently looking for an appropriate software package that allows me to do so. If...

30 January 2014 5,404 25 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View