Are desalted probes suitable for Northern Blot applications?

12 May 2017 1 9K Report

- would the signal be noisy with desalted oligos? Tm around 60C.

We tend to use P32 as a radioactive label for the probe.

the efficiency of prosphoramidite base addition is very high and oligos of hundreds of bases can now easily be made. The rate of fail additions is less than 0.5% so the amount of shortmers generated in oligos around 20-30 bases is very low and the shortmers will not anneal at the annealing temperature of 60c so I think it will be fine to use desalted oligos

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