How do i interpret this inconsistent mediation in my results ?

Dear all and any, I have run a mediation analysis on SPSS using the PROCESS extension (model 4). My results don't really make any intuitive sense, where my ID (availability of healthy fast foods,...

09 January 2021 7,812 2 View

What is the best technique to identify native bacteria groups present in soil?

I am lay in this subject. Is DNA sequencing the best way to identify the different types of soil bacteria present in soil samples? Ideally, I'd like to find a technique that allows me to identify...

29 September 2020 4,609 9 View

Someone has a suggestion to improve the purification of MMLV-RT protein?

I am working with a MMLV-RT (Moloney murine leukemia virus- Transcriptase Reverse). I am expressing in E. coli pTGroE and purifying in a Chelating Sepharose loaded with Ni2+. I am eluting the...

01 June 2020 4,105 4 View

How to make the student participate in the virtual classroom during the COVID-19 pandemic?

We are facing a difficult problem during the pandemic: the number of students attending virtual classes has decreased significantly at the undergraduate level.

20 May 2020 5,122 27 View

Purification of histag protein using HISTRAP HP (GE). What is the best lysis buffer and which protocol may be most suitable?

Purification of histag protein using HISTRAP HP (GE). I am working with insect cells (Tn5B) and my antigens are expressed in the nucleus. I am having difficulty lysing the cells because we have no...

10 March 2020 8,609 2 View

How to calculate Cronbach alpha for principal components?

I am using principal components to identify the main dimensions in a 19 item motivation scale. How can I assess the reliability of each component in SPSS?

17 February 2020 2,412 4 View

ERT Optimisation protocol sequence software-code for 3D unconventional electrode arrays?

Anyone know about any freely available software or code that can optimise protocol sequences for 3D unconventional electrodes setups, including borehole electrodes? The closest research I found...

15 November 2019 7,802 3 View

How to make a good and accurate test of spore viability?

Hello people! I have some gilled mushroom spore prints(on aluminum foil) one with 4 years and I need to have sure with they come to germinate on my culture media (PDA) or on my wheat spawn. What...

18 July 2019 1,658 3 View

How I can make a clean separation of fresh mycelium and culture medium on Petri dishes, to get fresh mycelium weight without medium influence?

I have some Pleurotus eryngii on Petri dishes with alternative solid culture medium and need to get fresh mycelium weight without the medium weight influence, which are the best and efficient...

09 April 2019 345 6 View

How to decrease adhesion of electrospun polymer fibers on usual metal collectors?

Any suggestions to reduce the adhesion of fibers to usual collecting metals such as aluminum, for easier recover of the fibrous membrane formed (or at least causing less damage)? So far, different...

30 January 2019 2,817 2 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View